If you are in masters split 1, are you in split2?

Regarding the road to ascention, are those in split 1, automatucally in split 2 and mid season? Ex invited orgs? Or foes it change, sorry im a little dumb

So for the road to Ascension:

It really depends on the Challengers' region. Some Challengers (like LATAM, JP, and OCE) adopts a relegation system where the bottom performing teams will have to go through the qualifier process once again. Some Challengers don't have any relegation at all, so they're safe for both Splits.

There's no mid-season international tournaments for Challengers, only Ascension.